The foundation for a healthy longevity!

Epigenetics controls how your genes work, affecting heart health, energy levels, immunity, and even the rate of aging. It acts like a switch: the right lifestyle - healthy food, sports, good sleep - can "turn on" genes that protect against disease and "turn off" those that increase risks. For example, stress or poor nutrition can increase inflammation, while an active lifestyle can strengthen cells. TruDiagnostic tests show how your habits affect these switches, helping you live longer and better.

In simple words: Epigenetics is like a remote control for your health. Eat your vegetables, move and get enough sleep - and your body will thank you by working better!

In scientific terms: Epigenetics regulates gene expression through DNA methylation, influencing processes of inflammation (e.g., IL-6 gene), metabolism, and cellular repair. Incorrect markers, such as hypomethylation of TNF-α, can increase the risk of autoimmune diseases, while correct changes induced by diet or sport reduce the risk of cardiovascular disease by 20-30% (Estruch et al., 2018).

Genes determine 20-50% of your health, and the rest depends on lifestyle - diet, physical activity, sleep and stress. Epigenetics shows how these factors regulate genes, allowing you to influence your well-being.

In simple words: Genes are like a recipe for your health, and you are the cook! Add good food, walks, sound sleep and a smile to your life and you'll glow with energy and live longer.

In scientific terms: Heritability of health is 20-50% for most chronic diseases (twin studies, 2020), whereas environmental factors modulate epigenetic markers, determining up to 80% of variability.

Nutrition (folate, polyphenols), physical activity (increases PGC-1α expression) and stress (increases cortisol) alter DNA methylation. For example, a diet with omega-3 can reduce methylation of inflammatory genes (such as IL-6), but the degree of change depends on dosage and duration (usually there is an effect after 6-12 months).

In simple words: What you eat, do and feel changes the way your genes work.

Scientific: Exogenous factors such as nutrients and stress hormones induce epigenetic changes by affecting promoter methylation and gene expression, such as IL-6, with effects observed after 6-12 months.

Yes, in some cases epigenetic changes can be transmitted through 1-2 generations, as shown by animal studies and limited data in humans (e.g., methylation of genes related to diet or stress). However, this transmission is unstable and is often reset during reprogramming of gametes (germ cells).

In simple words: Sometimes the habits of parents may affect children, but it is not forever.

In scientific terms: Transgenerational epigenetic heritability is observed when methyl tags are retained in gametes, but global demethylation during embryogenesis often eliminates these changes.

DNA methylation is the attachment of methyl groups (-CH₃) to cytosines at CpG-sites, suppressing or activating activity (gene expression).

In simple words: Methylation is like an on/off switch (dimmer) of genes that controls their operation (activity).

In scientific terms: Up to 80% of the more CpG-sites (sections) of human DNA are methylated to some degree, and as we age, the degree of DNA methylation at these different sites changes, which forms patterns. Researchers have successfully been able to link millions of these patterns to specific age and age-related health outcomes.

Epigenetic biomarkers are specific biological markers obtained by analyzing DNA methylation patterns in your genome.
The uniqueness of epigenetic biomarkers lies in their dynamism, as they are formed by the interaction of genes and environmental factors (nutrition, lifestyle, exposure to toxins and stress levels)
Epigenetic biomarkers provide unique insights into ongoing biological functions by predicting levels of nutrients, metabolites, and other important biochemical compounds.
Their main advantage is their ability to detect hidden imbalances and potential risks earlier than traditional laboratory biomarkers. This makes them a valuable tool in personalized medicine, nutrigenetics and preventive strategies for health maintenance.

Epigenetic biomarkers are not equal to clinical laboratory tests, but complement rather than replace them. On average, epigenetic biomarkers are superior to traditional laboratory biomarkers in both prediction and diagnosis:

• 62% of epigenetic biomarkers are more strongly associated with disease progression.
• 64% of epigenetic biomarkers are diagnostically more significant.
• Epigenetic biomarkers demonstrate 90% accuracy in detecting abnormalities from clinical norms.

Epigenetic biomarkers can predict the development of (help diagnose) 12 major chronic diseases: cardiovascular, type 2 diabetes, chronic kidney disease, cognitive impairment, and all-cause mortality.

Epigenetic biomarkers demonstrate 90% overall accuracy in detecting deviations from clinical norms; high specificity (94%) and sensitivity (76%) in clinical laboratory tests.

EBs provide a more stable and comprehensive view of your health over time, reflecting long-term impacts and trends rather than short-term fluctuations (as with lab markers).

Chronological (calendar) age is the number of years that have passed since we were born.

• This number is fixed and does not change according to improvement or deterioration in health and physical ability.

Biological (cellular) age is a calculation of how lifestyle, environmental factors, and genetics combine to influence the aging process.

• This is the actual state of your cells, which reflects how fast you are aging due to epigenetic changes, telomere shortening, and accumulation of damage in the body.

Yes, studies show that aerobic exercise increases PGC-1α expression by 20%, improving mitochondrial function, and a diet with resveratrol can activate SIRT1. An integrated approach (sport, diet, sleep) may slow biological aging by months or up to 1 year in a year, but the effects vary.

In simple words: A healthy lifestyle helps cells age slower.

In scientific terms: Physical activity and nutrients such as resveratrol modulate epigenetic markers and mitochondrial biogenesis, potentially reducing biological age by 0.5-1 year per year.

It correlates more strongly with disease risk (r≈0.75 for heart attack) and mortality than chronologic age (r≈0.6), a finding supported by studies including the Framingham cohort.

In simple words: Biological age is a better indicator of how you feel and what your risks are.

In scientific terms: Biological age as measured by epigenetic markers has a higher predictive value for chronic disease (r=0.75) than chronologic age, as shown in the Framingham Heart Study.

Chronic stress increases cortisol levels by altering gene methylation (e.g., NR3C1) and can shorten telomeres by 5-10%, accelerating biological aging by 1-2 years depending on the intensity and duration of stress.

In simple words: Stress makes cells age faster, but it can be controlled.

Scientifically speaking: Chronic stress induces hypomethylation of the NR3C1 gene, enhancing glucocorticoid signaling and shortening telomeres, which accelerates epigenetic aging.

Healthy eating, exercise, sleep and stress management slow down cell aging. Eat berries, nuts and omega-3 foods, exercise 30 minutes 5 times a week, get 7-8 hours of sleep and practice meditation or yoga.

In simple words: changes in your life - from a salad for dinner to going for a walk - help your cells stay young.

Scientifically speaking: Antioxidants and omega-3s reduce inflammatory gene methylation by 10-15% (Epigenetics, 2020), and physical activity and sleep improve mitochondrial function and DNA repair.

It is a scientific tool for estimating the biological age and aging rate of a person through methylation analysis (using algorithms that analyze epigenetic changes in DNA at CpG-sites). It is called a "clock" because it allows us to "measure" the aging rate of the organism by comparing it with the chronological age (the number of years lived according to the calendar).
Epigenetic clocks correlate with age-related diseases with 85% accuracy.

TruAge is the most advanced, accurate and scientifically validated epigenetic test that determines biological age, rate of aging and age of individual organs. Given that aging is the #1 risk factor for virtually all chronic diseases and death, slowing aging can significantly reduce these risks.

In simple words: The test shows you how your body is aging and gives you recommendations on how to slow down aging.

Scientifically speaking: TruAge uses high-throughput DNA methylation sequencing to assess epigenetic age and systemic aging using algorithms validated on the Dunedin and Framingham cohorts.

Published Algorithms
TruAge exclusively uses published, peer-reviewed and widely recognized algorithms (DunedinPACE, OMICmAge and SYMPHONYAge) developed with Duke, Harvard and Yale to ensure maximum transparency and scientific validity of results.

High accuracy and reproducibility
TruAge demonstrates stable and reproducible results in repeated testing (ICC intraclass correlation coefficient >0.98), confirming its reliability and scientific validity.

Comprehensive analysis of health and lifestyle factors
TruAge is based on data from more than 15 clinical studies conducted at the world's leading universities, including Yale. This allows for personalized, evidence-based recommendations to optimize health and longevity.

Multifaceted biological age assessment
TruAge provides 75 different age indicators, including total biological age, rate of aging, age of individual organs and systems, providing a detailed view of body condition.

The latest generation of biological clock technology
TruAge utilizes a third-generation aging clock, including DunedinPACE, that can determine the rate of aging with high accuracy, providing key data for preventative longevity strategies.

Maximum epigenetic analysis detail
TruAge analyzes approximately 1 million CpG DNA methylation sites, providing the deepest and most accurate assessment of epigenetic profile, making it one of the most detailed tests on the market.

Immune deconvolution for an accurate assessment of immune health
TruAge uses advanced immune deconvolution techniques to analyze changes in the composition of immune cells and their epigenetic patterns to provide an accurate assessment of the immune system and its impact on the aging process.

A detailed comparison with other tests is available at: https://drive.google.com/file/d/1Oa4nZuZs-cX-D9pNfYbLmxNO2Cu8BPg8/view

OMICmAge is the most advanced, accurate and scientifically proven algorithm for measuring biological age (how "old" or "young" your cells are compared to your passport age).

In simple words: This is an accurate way to know how old your cells are. For example, if you are 40 years old and your biological age is 30, your cells are in great shape! If it's higher, it's a signal that you can improve your health.

Scientifically speaking: The OMICmAge algorithm analyzes over one million DNA methylation sites, proteomic and metabolic data, reflecting the combined effects of lifestyle, environmental and medical factors on the body. The RMS error in predicting biological age is 1.5 years (surpassing Horvath's clock (3-5 years)).

The OMICmAge algorithm is the only epigenetic age counter that achieves almost 90% accuracy in predicting the risk of death and disease in both a five and ten years time span. If, for example, chronological age (year of birth) is used for prediction, the accuracy is only 75.6%.

The OMICmAge report also includes individual risks estimation for death, stroke, cancer, congenital heart disease, chronic obstructive pulmonary disease, depression, type 2 diabetes, methylation risk scores for various proteins, metabolites and clinical biomarkers.

The algorithms also calculate what the risks would be if biological age increased or decreased by one or more years.

The DunedinPace algorithm measures the body's rate of aging, its science-based conclusions are the most advanced and reliable on the market. By comparing the rate of aging to a standard calendar year, DunedinPace gives you a clear picture of whether your efforts to stay healthy are slowing or accelerating your biological aging.
The aging rate shows how fast your body "wears down" in a year, i.e. what is the rate of biological aging in years per calendar year based on the assumption that 1 calendar year = 1 biological year. For example, 0.8 means - slowing down. If it is greater than 1 (e.g. 1.1), aging is going faster and you should take care of yourself. The report shows which risks are associated with this and how to reduce them. The effect of slowing down depends on individual factors

In simple words: It's like an aging speedometer. It shows whether you are aging faster or slower than you should.

Scientifically speaking: DunedinPACE uses epigenetic markers to calculate the rate of aging correlated with phenotypic outcomes (r=0.78) based on the Dunedin Study longitudinal cohort.

The DunedinPace algorithm is a revolutionary approach to quantifying aging that shifts the focus from simply knowing your biological age to understanding the rate, or speed, at which you are aging. It's not just about how old your body is biologically; it's equally important to understand how fast you're moving toward aging. This knowledge is vital because slowing down the rate of aging can have a significant impact on your health, vitality, and prevention of chronic disease. By giving you a clearer picture of how fast you are aging, DunedinPace empowers you to make informed lifestyle choices that will help slow the aging process while striving for a healthier and more vibrant life. The rate of aging changes rapidly and has been shown to be lifestyle dependent, making it an ideal tool for evaluating the success of interventions.

The SYMPHONYAge algorithm is an organ-specific proxy for aging that assesses how different organs and body systems age both independently and in sync.

In simple words: Checks which organs are aging faster and for which, targeted interventions can do the most good.

In scientific terms: SYMPHONYAge uses an algorithm developed by Yale that analyzes 130 epigenetic markers, predicting age-specific organ changes with 85% accuracy.

TruHealth is a test designed to assess your current state of health at the cellular level using 110+ epigenetic biomarkers related to nutrition, metabolism, hormone balance, antioxidant defense, immunity, cognitive health, etc.

In simple words: The test checks if everything is okay with your health and what needs improvement.

In scientific terms: TruHealth is designed to help physicians identify underlying causes of dysfunction and correct clinical imbalances that prevent optimal health. This advanced diagnostic tool provides a systematic approach to help physicians help their patients overcome chronic diseases and lead healthier lives.

TruHealth provides deep insight into your body's functional nutrition and systemic health by testing +110 biomarkers, including +75 antioxidants, vitamins, minerals and fatty acids.

TruHealth measures the health of your cells in many systems including metabolism, immunity, NAD+ metabolism, mitochondrial health, neurocognitive health and more.

TruHealth provides an unrivaled assessment of your overall health beyond standard lab tests. Through personalized recommendations on nutrition, metabolic function, hormone balance, oxidative defense, and more, TruHealth gives you actionable recommendations to optimize your well-being at the cellular level. Our testing also identifies toxic exposures and assesses your body's ability to detoxify and neutralize these threats.

Imbalances in key health systems can contribute to the development of chronic diseases. If you suffer from any of the following conditions or care about your overall health, TruHealth's comprehensive testing can help identify the root cause of your issues: Depression, cardiovascular disease, cancer, infertility, polycystic ovarian syndrome, obesity and type 2 diabetes, chronic fatigue, weight issues, immune deficiencies, hypothyroidism, hypertension, autoimmune diseases, ADHD, malnutrition (especially in older adults), digestive disorders and malabsorption, athletic performance and recovery, physical injuries and healing.

Yes, TruAge + TruHealth analyzes aging and health (185+ biomarkers) from a single sample, providing a complete molecular profile.

In simple words: One test gives a complete picture - both how you age and how you live.

Scientifically speaking: the assay integrates 185+ epigenetic markers to assess aging and current physiological state from a single sample.

1. Epigenetic tests identify risks 5-10 years before symptoms appear, allowing you to act early. For example, changes in the CDKN2A gene can indicate cardiovascular problems.

In simple words:Atest is like a weather forecast for your health: it warns you of problems so you can avoid them.

Scientifically speaking: Epigenetic markers such as CDKN2A methylation predict cardiovascular risks with 75% accuracy.

Tests show you which diets or workouts are better for your body. For example, a vegetarian diet may reduce inflammation if your markers indicate such a need.

In simple words: The test will tell you what's good for you, whether it's more fish in your diet or yoga instead of running.

Scientifically speaking: Epigenetic profiles can tailor interventions by reducing inflammatory markers such as IL-6 by 10-15% with certain diets.

Yes, understanding your biological age motivates you to take charge of your health. People who recognize their results are 30% more likely to follow healthy habits.

In simple words: When you know your cells are younger or older than your passport age, it inspires change - from sports to quitting bad habits.

Scientifically speaking: Psychological research shows that epigenetic age feedback increases behavioral motivation by 30%.

Epigenetic tests (TruAge) analyze DNA methylation to predict risks 5-15 years in advance, while classical tests (blood tests) measure current indicators (glucose, cholesterol), reacting only to existing problems.

In simple words: Epigenetic tests "look" into the future, while conventional tests are "for today".

Scientifically speaking: Epigenetic tests identify molecular predictors of pathologies, whereas classical assays measure biochemical markers of the current condition.

Epigenetic biomarkers (e.g., GRIM-AGE) predict heart attack risk with a correlation of r=0.75 vs. r=0.65 for cholesterol because they reflect molecular causes rather than symptoms.

In simple words: They "see" what might happen, earlier than conventional tests.

Scientifically speaking: Epigenetic clocks such as GRIM-AGE have a higher predictive value for chronic diseases (r=0.75) by analyzing molecular drivers (Framingham, 2018).

No, classical laboratory tests (hemoglobin, C-reactive protein) do not "see" epigenetic changes such as CDKN2A methylation, which predicts disease 10 years before symptoms.

In simple words: Conventional assays show what is now, and epigenetic assays show what will be next.

Scientifically speaking: Classical tests are limited to measuring current biomarkers, whereas epigenetic tests analyze predictive molecular signatures such as CDKN2A methylation.

They use complex sequencing technologies, bioinformatics analysis and algorithms that require large databases and calculations, unlike simple biochemical and other methods of classical assays.

In simple words: It's like comparing an X-ray and an MRI - epigenetic tests are more complex and deeper.

Scientifically speaking: Epigenetic tests require high-throughput sequencing and machine learning, which significantly increases computational and laboratory costs compared to biochemical methods.

Every 3 months to monitor how lifestyle changes are affecting your biological age. This allows you to see the impact of your health interventions and make adjustments as needed.

In simple words: To observe progress.

Scientifically speaking: The designated testing frequency corresponds to the dynamics of epigenetic changes induced by lifestyle.

TruAge focuses on aging, while TruHealth focuses on health.

In simple terms: TruAge "watches" how you age and TruHealth watches how you live now.

Scientifically speaking: TruAge assesses the epigenetic clock and systemic aging, while TruHealth analyzes methylation associated with ongoing metabolic and immune processes.

The patient takes a capillary blood sample (finger prick) at any Alfa Diagnostica Testing Center. No preparation for the test is required. The turnaround time for the test varies from 2 to 3 weeks.

In simple words: It's a simple finger prick, like a sugar test, and the result in a couple weeks.

Scientifically speaking: The analysis is performed using Illumina microarrays for DNA methylation sequencing from capillary blood, with data processing in a CLIA-certified laboratory.

The tests are developed in conjunction with leading scientific institutions and are based on cutting-edge research. Analyzing more than 1,000,000 CpG strands of your DNA ensures highly accurate and reliable results.